Loss of SV2B decreases the expression of several synaptic vesicle proteins.

Eyes from two SV2B+/+ and two age-matched SV2B−/− mice were pooled and extracted after which equal amounts of protein processed for quantitative Western analysis as described under Methods. Each sample was run six times on a minimum of three blots. The ratio of average values (KO/wt) was calculated for each blot and the ratios averaged across blots. All proteins except SV2A and GAPDH were reduced compared to wild-type levels. Results are representative of another study done with eyes pooled from 4 mice of each genotype.