Binding Profiles of Myc and RNA Polymerase II in Rat Neonatal Cardiomyocytes

During the progression of cardiac hypertrophy, cardiomyocytes accelerate RNA biogenesis to meet increasing metabolic demands under exogenous hypertrophic stimuli. We have elucidated the quantitative kinetics of RNA in c-Myc (Myc)-mediated hypermetabolic analytical model using cultured neonatal rat cardiomyocytes. Chromatin immunoprecipitation and high-throughput sequencing demonstrate that overexpressed Myc binds to a specific set of genes directly in cardiomyocytes and recruits RNA polymerase II to these genes.在心肌肥厚（cardiac hypertrophy）的进程中，心肌细胞（cardiomyocytes）会加速RNA生物合成（RNA biogenesis），以应对外源性肥厚刺激物（exogenous hypertrophic stimuli）作用下不断升高的代谢需求。本研究利用培养的新生大鼠心肌细胞（cultured neonatal rat cardiomyocytes）构建了c-Myc（Myc）介导的高代谢分析模型，阐明了该模型中RNA的定量动力学特征。染色质免疫共沉淀（Chromatin immunoprecipitation）与高通量测序（high-throughput sequencing）实验证实，过表达的Myc可直接结合心肌细胞内的特定基因集，并将RNA聚合酶II（RNA polymerase II）招募至这些基因位点。