Imaging data for: Arp2/3 involvement in intercellular bridge membrane fluctuations and constriction during neural stem cell divisions

This dataset contains imaging data of Drosophila neural stem cells within larval brains, capturing cellular morphology and spatial organization. It accompanies the study “Arp2/3 involvement in intercellular bridge membrane fluctuations and constriction during neural stem cell divisions,” which investigates the mechanisms of intercellular bridge (ICB) remodeling during the final stages of neural stem cell division in the Drosophila larval brain. Unlike ICBs in cultured cells, which have flanking regions that thin as sibling cells move apart, neural stem cell ICBs lack flanking arms and constrict primarily at the midbody. Using super-resolution, full-volume live imaging, the study revealed dynamic plasma membrane fluctuations surrounding the ICB. Inhibition of the Arp2/3 complex, a key nucleator of branched actin filaments, reduced these fluctuations and impaired ICB thinning. These findings suggest that dynamic branched actin networks contribute to ICB membrane remodeling and help suppor..., Drosophila melanogaster was used as the experimental model organism in this study. All fly strains used are listed in the Key Resources Table of the associated publication. Third instar larvae of mixed sex were used for all experiments. Since dividing neural stem cells (NSCs) in the larval brain were the focus, larvae were selected at the wandering third instar stage to ensure active neuroblast proliferation. No sex-specific effects were analyzed, and sex-specific differences in NSC behavior have not been systematically assessed.
Flies were maintained on standard Bloomington recipe food at 25°C under a 12-hour light/dark cycle. For experiments involving UAS/GAL4-driven transgene expression, crosses were performed at 29°C to enhance GAL4 activity. A Worniu-GAL4 driver line was used to direct tissue-specific expression of UAS-controlled transgenes specifically in NSCs. Stocks were obtained from the Bloomington Drosophila Stock Center or from the laboratories indicated in the Key Resources..., # Imaging data for: Arp2/3 involvement in intercellular bridge membrane fluctuations and constriction during neural stem cell divisions

Dataset DOI: [10.5061/dryad.tmpg4f5d1](https://doi.org/10.5061/dryad.tmpg4f5d1)

## Description of the data and file structure

Imaging data for **Arp2/3 involvement in intercellular bridge membrane fluctuations and constrictions during neural stem cell divisions (**[https://doi.org/10.1101/2024.10.28.620743](https://doi.org/10.1101/2024.10.28.620743))

The deposited dataset consists of time-lapse microscopy image files in .tif format generated from live imaging experiments of *Drosophila* neural stem cells. These files can be opened and analyzed using standard bioimaging software such as Fiji (ImageJ) or Imaris.

The dataset includes multiple biological replicates for the experiments shown in the associated manuscript. Each .tif file represents an individual imaging experiment. File names correspond to specific figures or panels in the manuscript to f..., ,