S. coelicolor WL6268 R5- medium time course study

Antibiotic biosynthesis in Streptomyces species is controlled by a complex genetic and biochemical network of global and pathway specific regulators. Details of their precise interactions in mediating temporal and spatial expression of secondary metabolite genes remain poorly defined. In this study, we employed whole-genome microarrays to investigate the temporal transcriptome profiles of S. coelicolor A3(2) deltaSCO6268::kan mutant strain (WL6268) and compare it to the wild-type M145 strain. SCO6268 is a putative histidine kinase. The time-course study involved analysis of 7 samples (12h, 16h, 18h, 19h, 21h, 25h, 33h). Samples (19h, 21h, 25h, 33h) were analyzed on duplicate arrays (2 technical replicates). Sample 16h was analyzed on triplicate arrays (3 technical replicates). Genomic DNA of S. coelicolor wild type (M145) was used as a reference for all the arrays. cDNA was labeled with Alexa647 and genomic DNA was labeled with Cy3.