PVL does not alter global transcriptional profiles of USA300 and USA400.*

€Transcriptome analyses of SF8300 vs. SF8300Δpvl mutant strains were performed using custom Affymetrix GeneChips (RMLChip1) containing 3961 probe sets from eight different S. aureus strains (COL, EMRSA16, MSSA476, RF122, TSS, 8325, Mu50, and N315); and MW2 vs. MW2Δpvl using custom Affymetrix GeneChips (RMLChip3) with 99.3% coverage of genes from MW2 (2613 probe sets of 2632 ORFs; the remaining 0.7% are represented by identical probe sets from other staphylococci). Note that RMLChip1 does not contain probesets for lukS-PV and lukF-PV, which was subsequently assayed by TaqMan real-time RT-PCR (Table 2). Table displays only probesets that met standard criteria required for differentially-expressed genes (>2-fold change in transcript levels in the wild-type vs. Δpvl strain, and Pt test).¥spc, encoding spectinomycin adenylyltransferase (detected only in SF8300Δpvl by RMLChip1; probe set absent in RMLChip3); lukF-PV and lukS-PV, encoding PVL (detected only in MW2 by RMLChip3; probe sets absent from RMLChip1); set, encoding an enterotoxin homolog (MW0052), 2.4 fold-change; lukE (MW1768), encoding leukocidin E, 2.1 fold-change; and plc (MW0070), encoding 1-phosphatidyl-inositol phosphodiesterase precursor, 2.6 fold-change.