Single-cell transcriptome profiling of Sox2-low iPS cells. Single-cell transcriptome profiling of Sox2-low iPS cells

Single-cell RNA-seq was performed on homozygous Sox2 knockout induced pluripotent stem (iPS) cells, where residual Sox2 expression is observed from incompletely silenced retroviral transgenes. These were compared to Sox2-low iPS cells rescued by exogenous Sox2 transgenic expression, and wild-type iPS cells to assess the lineage expression profile of Sox2-low cells and degree of transcriptional heterogeneity in each population.