transcript analysis of strains with or without plasmid containing 40 genes in three different culture conditions. transcript analysis of strains with or without plasmid containing 40 genes in three different culture conditions

Purpose: To investigate the influence of the 40 genes on plasmid, we compared the transcript varies between the engineered strains and the wild-type strain in synthetic medium with different carbon sources (i.e., glucose, galactose and ethanol/glycerol media). Methods: Total mRNA profiles of the engineered strains and the wild-type strain were generated by deep sequencing, in triplicate, using BGIseq500. The sequence reads that passed quality filters were analyzed. Results: RNA sequencing results revealed that the transcript patterns were influenced dramatically by the carbon sources and there was no significant difference between the wild-type and engineered strains (that is, any change amounted to less than 10%) Overall design: Total mRNA profiles of the engineered strains and the wild-type strain which grew to log phase and collected.