Assembly of Puccinia triticina race 9 genome

Puccinia triticina (Pt) causes wheat leaf rust and is an obligate biotrophic fungus, and because of its life cycle and mode of reproduction, is difficult to study genetically. To identify Avr genes in Pt, a F2 population of fifty-seven progeny was generated from the sexual cross of race 9 (SBDG) and race 161 (FBDJ) on the alternate host Thalictrum speciosissimum under controlled conditions. The population segregated for avirulent/virulent traits screened at the seedling stage on thirteen single gene resistance lines in the wheat host cultivar Thatcher background. The genomes of the parents, F1, and progeny were sequenced and mapped onto an assembled parental race 9 phased haplotype B genome, resulting in 21,154 high-quality SNP markers suitable for genetic mapping of the F2 population.