RNA-Seq on common bacterial pathogens in the presence vs absence of a variety of antibiotics. Two susceptible and two resistant isolates were exposed to antibiotics in laboratory culture, total RNA wa
As the efficacy of conventional antibiotics continues to decline due to antibiotic resistance, there is an urgent need for alternative antimicrobial strategies. Polyhexamethylene biguanide (PHMB), a c
Using CRISPR/Cas9-assisted genome-editing tools, we constructed 68 efflux pump mutants of M. abscessus and investigated their roles in drug resistance and virulence. We found that different efflux pum
