Exome sequencing of Lmo2-driven murine T-ALL

To explore the nature of co-operating genetic events in murine Lmo2-driven T-cell acute lymphoblastic leukemia (T-ALL), we serially transplanted thymocytes from preleukemic (8-10 week old) Lmo2-transgenic mice into irradiated recipients, with secondary recipients then left to develop leukemia. Exome sequencing was then performed using tail DNA (as a germline reference), preleukemic thymocytes (n=4) and leukemia derived from secondary transplants (n=17 in total from 2 donors). These data were then analysed to determine (i) the genomic landscape of Lmo2-induced T-cell leukemia and (ii) the ontogeny of secondary mutations, to distinguish early- and late-arising events.