Comparing methods of human precision cut tissue slice culture - tissue slice viability and functional markers data

This study examines the viability and functionality of hPCTS using different tissue culture formats. hPCTS were cultured for 15 days using an organotypic polytetrafluoroethylene Millicell insert, a CELLBLOKS® hydrophilic polyethylene terephthalate flow system plate or without an insert. Viability was assessed through MTS assays, while functionality was determined by measuring urea and albumin secretion across the 15 days in culture.1. File Name: Viability Data NC3Rs paper.xlsx - Visibility: Anyone - Content type: Data - Description: MTS viability data for human precision cut tissue slices (hPCTS) cultured over 15 days using three different culture methods (no insert, Millicell insert, CELLBLOCKS®). - Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet - File size: ~51 kB - License: Creative Commons Attribution-Noncommercial-Share Alike 4.0 2. File Name: Urea and Albumin Data NC3Rs paper.xlsx - Visibility: Anyone - Content type: Data - Description: Percentage change in urea and albumin secretion into media from day 3 in hPCTS cultured over 15 days. - Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet - File size: ~19 kB - License: Creative Commons Attribution-Noncommercial-Share Alike 4.0