Initial rates with different substrate concentrations were measured In vitro, and kinetic properties (KM, Vmax and kcat) were determined by non-linear regression analysis using GraphPad 5 software. Va
aValues represent means of three replicates, and ± standard errors are reported.bThe activity of each substrate was determined by measuring absorbance at specified wavelengths according to the relativ
Structure of the E192N mutant of E. coli N-acetylneuraminic acid lyase in complex with pyruvate in crystal form III Descriptor: N-ACETYLNEURAMINIC ACID LYASE, PENTAETHYLENE GLYCOL, PYRUVIC ACID Author
Structure of Rex8A from Paenibacillus barcinonensis complexed with xylose. Descriptor: GLYCEROL, Reducing-end xylose-releasing exo-oligoxylanase Rex8A, beta-D-xylopyranose Authors: Jimenez-Ortega, E,