IRF-1 expressed in the early blastocyst inner cell mass of pigs enhances the pluripotency of induced pluripotent stem cells

Naïve porcine stem cells is supposed to rely on the regulation of unique transcription factor in the early inner cell mass. The expression of Interferon regulatory factor 1 (IRF-1) were detected in aggregation in d6~7 somatic cell nuclear transfer blastocysts, which was supposed to regulate the pluripotency of naïve state. But IRF-1 is reported to be involved in the response to viral infections and initiate a rapid proinflammatory response, while the function of IRF-1 on pluripotency need to be explored. Then the heterogenicity of IRF-1 was associated with pluripotency in pig induced pluripotent cells and overexpression of IRF-1 promoted the gene expression profile of pluripotent genes as would be expected when cells approach the naïve state Overall design: In order to determine how IRF-1 expression affects RNA expression, we performed high-throughput RNA sequencing on overexpression and WT cells. These data showed that overexpression of IRF-1 in pig induced pluripotent cells promoted the expression of pluripotency genes related to the naïve state and down-regulated genes related to differentiation processes. Then In order to detect the target genes of IRF-1, CHIP-seq was conducted using an anti-flag antibody and the chromatin of IRF-1-flag overexpressing cells. The captured genes were used to enrich for KEGG-pathways and the most enriched biological pathways were related to initiate immunity and adaptive immunity, as expected