Effect of PD-1 immune checkpoint in Alzheimer's disease transgenic mice

We examined the brain's choroid plexus and myeloid cell populations isolated from the brain of 5XFAD Alzheimer's disease transgenic mice following PD-1 blockade Choroid plexus samples and myeloid cell populations were isolated from the brain of 5XFAD mice following PD-1 blockade, and sequenced. For choroid plexus samples, 5 mice were treated with anti-PD-1, 5 with IgG control, and 4 were left untreated. For the myeloid cells samples, myeloid cells sorted from the brains of 5XFAD mice according to a gating strategy that seperate microglia (CD11b+CD45-low) and monocytes-derived macrophages (CD11b+CD45-high).