Transcriptional profiling of mutants of FdC2 (At1g32550) in Arabidopsis thaliana

Two RNAi knock-down lines for At1g32550 were generated and protein content confirmed by western blotting. Individuals of wild type, one severe (line 8) and one mild (line 1) knock-down line were grown to four weeks old and then total RNA extracted from 2 h into the light period. Total RNA was extracted and then sent to OakLabs for hybridization and analaysis The Agilent Arabidopsis chip was enriched for chloroplast transcripts in a custom array