Comprehensive comparison of female germ cell development in vitro and in vivo identifies epigenetic gene regulation crucial for oocyte development and embryonic competence [scRNA-seq]

Single cell transcriptomics and functional assessment in a stage-specific manner provides a comprehensive comparison of entire female germ cell development in vitro and in vivo. Overall design: To identify possible causes underlying the overall low oogenic and embryonic competence of in vitro developed oocytes, we performed extensive comparative transcriptome analysis between in vivo and in vitro generated germ cells, at different stages of their development.