Text S1 - Proteomic Selection of Immunodiagnostic Antigens for <i>Trypanosoma congolense</i>

Figure S1. SDS-PAGE with Coomassie blue staining of purified recombinant proteins. The names of the expressed proteins appear above each respective gel lane. The bands indicated by arrow heads are: A, Tc38630; B, degradation product of Tc38630; C, E. coli Ef-Tu (co-purifying contaminant); D, Tc29290. These identities were confirmed by tryptic digestion and mass spectrometry. Figure S2. Assessment of Tc38630, Tc29290 and Tc51750 ELISA assays with pre- and post-infection calf sera. (A) ELISA plates coated with the three recombinant proteins were tested with pre-infection (day −7; n = 40) and post-infection (day +28; n = 40) calf sera. The data were plotted on RoC curves of specificity against selectivity (1-specificity). The output statistics show a sensitivity and specificity of 90% and 94.2% for Tc38630, 80% and 67.3% for Tc29290 and 82.5% and 67.3% for Tc51750, respectively. Table S1. Antigens selectively recognised by T. congolense infection IgG. The antigens are ordered by infection : control LC-MS/MS intensity and coloured coded according to their by LC-MS/MS intensities: black bold >1000, black >60, grey <60. Only antigens uniquely bound by infection IgG to or with high intensity (>1000) and an infection : control ratio >100 were assigned identities. Those that failed to yield soluble protein in expression and purification trials are marked with ▪. Those selected but untested in expression trials are marked with white rectangle (vectors available on request) and those purified successfully are marked white rectangle. Table S2. Amino acid sequences of the seven antigen domains successfully expressed in E. coli. Differences in sequence to those in the TriTrypDB are highlighted in red, these are probably due to strain variation. (DOC)