Supplemental Material for Nageshan, Krogan, and Cooper, 2024

Supplementary information:

Table S1: Unprocessed list of the genes in the top 25th percentile of strength of interaction with the indicated query strains, and corresponding NGR values. Note that NGR value of a target gene represents the effect of that gene deletion on growth at 19°C relative to 30°C in the query mutant background (see methods). Sheet 1 shows a list of positive genetic interactors with taz1D, while Sheets 2-5 show negative genetic interactors with the indicated query mutant.

Table S2: List of genes that show negative genetic interaction specifically with taz1D at 30°C. The first sheet shows gene information along with respective NG and Log2 NG values (see Methods). Sheet 2 shows the GO tags for the genes from Sheet 1. Sheet 3 is a summary of Sheet 2, indicating the number of genes corresponding to each GO tag. In addition, Sheet 4 in Table S2 provides a description of the GO analysis column headings of the table represented in Sheet 2.

Table S3: List of top 25th percentile suppressors of taz1D c/s. The sheet format follows that of Table S2, except that NGR and Log2NGR are presented (see Methods). The high confidence hits determined by the 2-standard deviation method are highlighted in purple in Sheet 1. GO tags and GO analysis summaries for the high confidence hits are presented in Sheets 2 and 3, respectively.

Table S4: List of genes whose deletion reverts the ability of rif1+ deletion to suppress taz1D c/s. The sheet format follows that of Table 2, with NGR values. These genes were identified as explained in Figure 3a and b.

补充信息：
表格S1：与指定查询菌株相互作用强度处于前25百分位数内的基因未处理清单，以及相应的NGR值。请注意，目标基因的NGR值表示该基因缺失对查询突变背景中19°C相对30°C生长的影响（参见方法）。第一页显示了与taz1D呈正遗传互作作用的基因清单，而第二页至第五页则展示了与指定查询突变体呈负遗传互作作用的基因。
表格S2：在30°C下与taz1D特异性呈现负遗传互作的基因清单。第一页展示了基因信息及其相应的NG和Log2 NG值（参见方法）。第二页显示了第一页中基因的GO标签。第三页是对第二页的总结，表明对应于每个GO标签的基因数量。此外，S2表格中的第四页提供了表格2中所示表格的GO分析列标题的描述。
表格S3：taz1D c/s的顶级25百分位数抑制剂的清单。页面格式遵循表格S2，除了NGR和Log2NGR以（参见方法）所示。在第一页中，通过2倍标准差方法确定的置信度高的击中项以紫色突出显示。高置信度击中项的GO标签和GO分析总结分别展示在第二页和第三页。
表格S4：删除其基因可逆转rif1+缺失对taz1D c/s抑制能力的基因清单。页面格式遵循表格2，包含NGR值。这些基因的识别方法已在图3a和b中解释。