Transcriptional difference between wild-type and zip-3(gk3164). Caenorhabditis elegans

ATFS-1 has been shown to regulate transcription of mitochondrial chaperone genes such as mtHsp70/hsp-6 and hsp-60 in response to mitocondrial stress. To identify the entire ATFS-1-mediated response, we compared the transcript profiles from wild-type and atfs-1(tm4525) worms raised in the absence and presence of mitochondrial stress. We used microarrays to identify genes regulated by ZIP-3 Overall design: RNA samples were prepared from wild-type(wt) and zip-3(gk3164)(mutant) worms fed either control(RNAi). Worms were synchronized by bleaching, raised in liquid culture and harvested at the L4 stage. Each experiment was performed in triplicate indicated as 1,2 and 3.