Novel eINTACT system dissects bacterial exploitation of plant osmosignalling to enhance virulence

SourceData_Fig2f: Relative expression of XopD, PR1, PR2, OSCA1.1 and HYL1 after XopD was induced by β-estradiol in Arabidopsis seedlings, relative to their expression in DMSO-treated seedlings. SourceData_Fig3b: Disease index scores (0, no symptoms; 0.5 to 1.5, weak chlorosis; 2 to 3, strong chlorosis) from n=40 each of osca1-1 or Col-0 leaves infected with Xcc* from 8 individual plants, at 7 DPI. SourceData_Fig3c: Bacterial population density in n=23 osca1-1 or n=24 Col-0 leaves infected with Xcc* from 8 different plants, at 7 DPI. SourceData_Fig3e: Relative expression of OSCA1.1 in Col-0 leaves infiltrated with Xcc∆xopD*dTALE#1 (dTALE#1) and Xcc∆xopD*dTALE#2 (dTALE#2), relative to its expression in Xcc∆xopD*vec2 (vector) infiltrated leaves, at 1 DPI. SourceData_Fig3h: Bacterial population density in Col-0 leaves inoculated with Xcc∆xopD* (-XopD) and Xcc* (+XopD) strains that deliver empty vector (vector), dTALE#1 or dTALE#2, at 7 DPI. SourceData_Fig4b: RT-qPCR analysis of RD29A expression in nuc+XopD relative to its expression in nuc-XopD. SourceData_Fig4c: Relative abundances of miR159a in nuc-XopD and nuc+XopD compared to its abundance in total nuclei of mock-inoculated leaves. SourceData_Fig4e: The water loss rate of detached Xcc* (+XopD) and Xcc∆xopD* (-XopD) infected leaves at 7 DPI. Data are presented as mean values +/- SD (error bars) from n=8 leaves from 4 different plants. SourceData_Fig4f: Bacterial population density in symptomatic leaf tissues inoculated with Xcc* (+XopD) and Xcc∆xopD* (-XopD) at 7 DPI. SourceData_ED_Fig2c: Bacterial population density in n=12 leaves of the eINTACT reporter line inoculated with Xcc*AvrBs3 and n=12 wild-type Col-0 leaves inoculated with Xcc*vec1, at 7 DPI. SourceData_ED_Fig4: RT-qPCR analysis of relative expression of NRPD1A, DDM1, HYL1, DWA1 and OSCA1.1 in Xcc∆xopD*AvrBs3 (-XopD) and Xcc*AvrBs3 (+XopD) inoculated leaves of the eINTACT reporter line at 5 DPI. SourceData_ED_Fig6c: Disease index scores (0, no symptoms; 0.5 to 1.5, weak chlorosis; 2 to 3, strong chlorosis) from n=30 each of Col-0, osca1-1 or Pro35S:OSCA1.1/osca1-1 leaves infected with Xcc* from 8 individual plants at 7 DPI. SourceData_ED_Fig6d: Bacterial population density in n=12 each of Col-0, osca1-1 or Pro35S:OSCA1.1/osca1-1 leaves infected with Xcc* from 4 different plants at 7 DPI. SourceData_ED_Fig8: ABA levels in Xcc*AvrBs3 and XccΔxopD*AvrBs3 infected leaves, at 5 DPI. SourceData_ED_Fig9: Bacterial population density in Arabidopsis leaves inoculated with Xcc∆xopD* or Xcc*, at 1, 3, 5 and 7 DPI.