Leaf transcriptome sequencing of diverse Pooideae species

RNAseq was performed on diverse grass species from the genera Achnatherum, Aegilops, Agropyron, Avena, Brachypodium, Briza, Cynosurus, Echinaria, Holcus, Hordeum, Koeleria, Lolium, Melica, Phalaris, and Poa that vary for their reaction to wheat stripe rust (Puccinia striiformis f. sp. tritici) to identify NLRs and their relative expression level. Accessions were sequenced using 150 bp paired end reads. RNAseq read quality was assessed and trimmed using Trimmomatic (version 0.36) with the parameters ILLUMINACLIP:2:30:10, LEADING:3, TRAILING:3, SLIDINGWINDOW:4:15, and MINLEN:100. Transcriptome assembly was performed using Trinity (version 2013-11-10) with default parameters.