Genotyping-by-sequencing data of the Wild Barley Diversity Collection

To identify new sources of stem rust resistance for barley, we evaluated the Wild Barley Diversity Collection (WBDC) (314 ecogeographically diverse accessions of Hordeum vulgare subsp. spontaneum) for seedling resistance to four pathotypes (TTKSK, QCCJB, MCCFC, and HKHJC) of the wheat stem rust pathogen (Puccinia graminis f. sp. tritici, Pgt) and one isolate (92-MN-90) of the rye stem rust pathogen (P. graminis f. sp. secalis, Pgs). The WBDC accessions were genotyped using Genotyping by Sequencing (GBS) technology. DNA was extracted using the Mag-Bind Plant DNA Plus kit from Omega Bio-tek (Norcross, GA), following the manufacturer's instructions. Genomic DNA was quantified using the Quant-iT PicoGreen dsDNA Assay Kit and normalized to 20 ng µl-1. GBS libraries were created using the Pst1-Msp1 restriction enzyme combination as described in Poland et al. (2012). The samples were pooled together at 48-plex to create pooled libraries, which were then sequenced on Illumina Hi-Sequation 2500.