Transcriptome of U251 cells knocking down RPL13

RPL13 was identified to be downregulated in brain of Alzheimer's disease (AD). To explore the involved pathways in response to RPL13 reduction, we knockdownRPL13 using siRNA in U251 cell line with a stably expressed mutant APP (K670N/M671L, U251-APP cells). RNA-seq (by IlluminaHiseq 4000) and following analyses were then performed to detect genes whose expression were influenced by RPL13 knockdown. Overall design: RPL13 inteference were performed in U251 cell with a stable expression of mutant APP 128 (M671L) (U251-APP cells) with biological triplicates for each condition (siNC, and RPL13). Differential expression genes were obtained by comparing gene expression levels in RPL13 knockdown (si-RPL13) with control cells (siNC).