A landscape of circular RNAs expression in the castration-resistant prostate cancer

Prostate cancer is one of the major cancers that seriously affect men's health. It has high morbidity and high mortality, but there is still no ideal molecular markers for the diagnosis and prognosis of prostate cancer. Castration-resistant prostate cancer is associated with wide variations in survival. To determine whether differentially expressed circRNAs in plasma exosomes can be used as a novel biomarker for castration-resistant prostate cancer prognosis, we performed high-throughput circRNA sequencing on 15 pairs of plasma exosomes from 30 metastatic castration-resistant prostate cancer patients, with or without early progression, to screen differentially expressed circRNAs. Overall design: We performed deep RNA-sequencing on ribosomal-depleted RNA isolated from 15 pairs of plasma exosomes from 30 independent patients with castration-resistant prostate cancer, with or without the presence of metastases, using Illumina X Ten. Patients were paired according to their age and clinical indicators.