Neuronal Hyperactivity due to Loss of Inhibitory Tone in APOE4 Mice Lacking Alzheimer’s Disease-like Pathology

Metabolite profiling was performed on metabolites extracted from the entorhinal cortex and primary visual cortex of 14-15 month old APOE3/3, APOE3/4 and APOE4/4 mice. Metabolites were run on a TOF Mass Spectrometer using an ANP column. Initial analysis was done in an untargeted manner, and processing was done to determine the differentially expressed metabolites based on their mass and retention times. Further analysis was then performed to assign identities to the differentially expressed metabolites using a database of biologically-relevant metabolites whose standards had been run under identical conditions as the samples in the study.