Role of Kindlin-2 in breast cancer immune evasion

Kindlin-2 (K2) plays a major role in driving TNBC tumors progression and metastasis through the regulation of several hallmarks of cancer. We have employed a single cell RNA sequencing approach using 10x Genomics scRNAseq to study the role of Kindlin-2 in immune evasion of TNBC tumors. Tumors derived from control 4T1 cells or their K2-KO derivatives grown in syngeneic immune competent BALB/c mine werw subjected to scRNAseq to indentify the efect of loss of K2 on the tumor immune environment. Control or K2-KO 4T1 cells were injected in the mammry fat pads of female BLB/c mice.tumors reach 150 mm3, tumors wer excised, followed by disociation into sigle cell supspension then analyzed using scRNAseq.