Paired CRISPR screens to map gene regulation in cis and trans (CUT&RUN)

In this study, we develop a scalable approach that pairs discovery of cis- and trans-acting elements to systematically decipher the gene regulatory network. We explore the regulation of the immune checkpoint PD-L1 under both basal and IFN?-stimulated contexts. PD-L1 expression on tumor cells serves as a clinically-actionable diagnostic for immune checkpoint blockade therapy across multiple cancer types. Overall design: Cleavage Under Targets & Release Using Nuclease (CUT&RUN) for SRF, BPTF, and H3K4me3 in BxPC3 human pancreatic adenocarcinoma cells