Signaling signature specific for effective ECFC+MSPC co-transplantation in antibody microarrays.

Signaling molecules expressed significantly in co-transplants of ECFC+ MSPC in vivo compared to that of equal numbers of ECFC only, MSPC only, Matrix only without cells [all harvested 24 h after transplantation] and ECFC+MSPC mixture in vitro are shown. The total number of microarrays is four with each target antibody determined in duplicate (1,760 spots per condition). ↑ arrows indicate up-regulation and ↓ arrows down-regulation of the target in ECFC+MSPC in vivo compared to the four different groups. Z-ratio of more than ±1.2 was considered to be significant. The results have been submitted to Gene Expression Omnibus (accession number GSE45896). For a complementary list of the expressed signaling molecules see the Table S1.
Casp4: Caspase 4 (ICH2 protease, ICE(rel)-II); Casp7: Caspase 7 (ICE-like apoptotic protease 3 (ICE-LAP3), Mch3); CAMK2b: Calcium/calmodulin-dependent protein-serine kinase 2 beta; DNAPK: DNA-dependent protein kinase catalytic subunit; Casp2: Caspase 2 (ICH1 protease); Casp12: Caspase 12 (mouse); CAMK2g: Calcium/calmodulin-dependent protein-serine kinase 2 gamma; ErbB2: ErbB2 (Neu) receptor-tyrosine kinase; DGKz: Diacylglycerol kinase zeta; Nek2: NIMA (never-in-mitosis)-related protein-serine kinase 2; Hsp27: Heat shock 27 kDa protein beta 1 (HspB1); DAXX: Death-associated protein 6 (BING2); HO-1: Hemeoxygenase 1; CAMK2a: Calcium/calmodulin-dependent protein-serine kinase 2 alpha; Casp5: Caspase 5 (ICH3 protease, ICE(rel)-III); Casp6: Caspase 6 (apoptotic protease Mch2); PACSIN1: Protein kinase C+casein kinase substrate in neurons protein 1; Erk 1/2: Extracellular regulated protein-serine kinase 1; CAMK2d: Calcium/calmodulin-dependent protein-serine kinase 2 delta; FAK: Focal adhesion protein-tyrosine kinase; FGFR1: Fibroblast growth factor receptor-tyrosine kinase 1; EphA1: Ephrin type-A receptor 1 protein-tyrosine kinase; P53: Tumor suppressor protein p53 (antigenNY-CO-13).