Bulk-RNA seq for Macrophages sorted from mouse lungs post influenza infection

To investigate whether SPARCL1 affects lung macrophage polarization in vivo RNA sequencing (RNA-seq) of isolated lung macrophages (CD45+Ly6G-CD64+F4/80+) on day 20 post-influenza infection was used to further examine the consequences of EC overexpression of Sparcl1. Overall design: FACS-sorted lung macrophages (CD45+/Ly6G-/CD64+/F4/80+) from ECSparcl1-OE and WT mice on D20 post influenza infection were used for sequencing.