Candida tropicalis strain:No.1254 Transcriptome or Gene expression

Here we report the enhancement of both cellular permeability and cell growth rate of Candida tropicalis after treatment with the ultrasonic irradiation of low intensity using a mode of sweeping frequency (UILS) genenated by a self-developed ultrasonic device in our lab. After the ultrasonic treatment, remarkable biomass enhancement of the yeast was observed; the hyphae became significantly longer; the seeped cellular protein and nucleic acid from the yeast increased and the cellular Ca2+ content became lower. Illumina transcriptome sequencing showed that the ultrasonic treatment affected the expression of genes involved in diverse cellular components, biological processes and molecular functions. RT-PCR and Western blotting further confirmed the up-/down-regulation of genes in the ultrasound-treated yeasts. The optimal conditions of the ultrasonic treatment for the maximum biomass addition were determined as follows: the yeast was treated for 1 hour at the mid logarithmic phase, the frequency was 28±2 kHz and the power density was 120 W/L. Under these conditions, the Candida tropicalis biomass increased by 142.5% compared with the untreated yeast.