Major virulence factors of EC958 and other sequenced UPEC/ExPEC strains.

1Strain name and unique locus tag for reference sequence used in BLASTn search of EC958 and complete UPEC genomes (accession number provided for queries not found in complete genome sequences); + or − indicates presence or absence, respectively; unless otherwise indicated, genes share >90% nucleotide identity with reference sequence and presence was confirmed using ACT comparison.2Indicated gene found in 91–100% of O25b-ST131 E. coli isolates from two previous studies [6], [7].3CFT073, UMN026 and 536 have two agn43 alleles whereas UTI89, IHE3034 and EC958 each have only one; Although the EC958 agn43 allele is similar to both CFT073 alleles, the UTI89 and IHE3034 alleles are distinct with limited amino acid similarity throughout their central domains (data not shown).4Truncated gene or putative pseudogene; in EC958 these are all caused by assembly breaks and may be bona fide full-length genes.5Gene shares 6There are two paralogous copies of ompT found in IHE3034; CFT073, UTI89, 536 and EC958 carry only a single copy corresponding to IHE3034 ECOK1_0575, whereas UMN026 and IAI39 contain only a single copy corresponding to IHE3034 ECOK1_0571.