Transcriptome analysis of stomatal lineage ground cells (SLGCs)
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https://www.ncbi.nlm.nih.gov/sra/SRP192828
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Purpose: Identify SLGC cell fate by transcriptomes Methods: Cells expressing SLGC markers (BASLp:BRX-YFP, BASLp:myrBRX-YFP and myrBRXp:myrBRX-YFP) and stomatal commitment stage marker (MUTEp:MUTE-YFP) were collected by FACS. 9dpg plants were used for protoplasting and subjected to cell sorting. Around 20,000/sample were cellected and used for library preparation. Results: SLGCs are renriched in mitotic genes. In addition, top candidates, DEK and MYB16 proteins function in SLGC cell divisions. Conclusions: SLGCs are division competent cells and the coordination of cell cycle and transcriptional regulation though DEK is important for maintaining the transition (SLGC) state during stomatal development. Overall design: SLGC mRNA profiles of 9dpg seedlings were generated by deep sequencing, in duplicates, using Illumina HiSeq.
创建时间：
2021-05-20
