Z' factors, false positive and negative rates were calculated from test plates of U2OS cells treated with taxol (0.1 and 2 µM, n = 20 for each) or 0.2% (n = 62) DMSO in a random distribution.
* “2-1” shows the screening efficiency for No. 2 library after one round of second-step selection.** “2-2” shows the screening efficiency for No. 2 library after two round of second-step selection.Scr