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mRNA transcriptome analysis of hypoxia with shSETDB1 in hela cells. mRNA transcriptome analysis of hypoxia with shSETDB1 in hela cells

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NIAID Data Ecosystem2026-03-12 收录
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Purpose: The goals of this study are to compare NGS-derived Hela with shSETDB1 and shcontrol in hypoxia transcriptome profiling (RNA-seq) to quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods Overall design: Methods: mRNA profiles of shControl hela and shSETDB1 with or hypoxia (1% O2) for 12 hours were generated by deep sequencing, in duplicate. GSEA was performed, qRT–PCR validation was performed

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2021-07-02
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