Additional file 3: of Three-dimensional evaluation of murine ovarian follicles using a modified CUBIC tissue clearing method
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GFP immunohistochemistry using ovaries of CAG-EGFP mice. Female CAG-EGFP mice were fixed with the transcardial perfusion of 4% PFA. Sections were stained with Hoechst 33,342 and anti-GFP antibody to reveal the expression of EGFP protein. Note that although GFP fluorescence was undetectable in granulosa cells, the expression of immunoreactive EGFP protein was detected. Scale bars, 100Â Îźm. ( 668 kb)
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2018-08-03
