The non-coding RNA miR-17∼92 is a central mediator of T cell costimulation [II]

To unravel the molecular mechanism underlying miR-17∼92 costimulatory function, we performed RNA-seq on CD4+ T cells from mice lacking miR-17∼92 in T cell, wildtype mice, mice overexpressing miR-17∼92 in T cells , mice with a constitutive knockout of CD28, and rescue mice, in naïve state or activated vitro for 24h. We compared mice from 5 genotypes: CD4cre.miR-17∼92lox/lox (designated T1792Δ/Δ hereafter); wildtype (designated wt hereafter); CD4cre.Rosa26loxSTOPloxCAG-miR-17∼92Tg (designated T1792tg/tg hereafter); B6.CD28-/- knockout (designated CD28-/- hereafter); and B6.CD28-/-.CD4cre.Rosa26loxSTOPloxCAG-miR-17∼92Tg (designated rescue hereafter). Naïve CD4+ T cells were stimulated in vitro for a duration of 24 hours.