Transcription Factor (TF)EB mediates a mTORC1OFF-induced ECM degradation program of breast cancer cells

We show that mTORC1 repression in breast cancer cells potentiates an invadopodia-based metastatic program Overall design: mTORC1 is central to nutrient sensing and controls endolysosomal biogenesis and exocytosis. To identify genes dysregulated upon mTORC1 repression, we silenced the mTORC1 tethering factor, RagC, in the human MDA-MB-231 breast cancer cell line. Two independent siRNAs againt RagC were used. We then performed RNA-sequencing to have a comparative analysis of gene expression.