Prokaryotic community structure in upland red soil under log-term fertilization

Addition of organic materials and liming is suggested to be feasible practices for mitigating red soil degradation. however, how organic materials improved soil quality and affected the aggregation and shift in prokaryotic community structure in a cultivated red soil is not well understood. In this project, we evaluate the relationship between soil quality, aggregation and the prokaryotic community in soils subjected to 27 years of inorganic and organic fertilization using high-throughput sequencing. The field experiment in subtropical upland in Yingtan, Jiangxi Province, China involved seven treatments: no fertilization (control), inorganic NPK fertilizer (N), inorganic NPK fertilizer plus liming (CaCO3) (NL), and inorganic NPK fertilizer plus peanut straw (NPS), rice straw (NRS), fresh radish (NFR), and pig manure (NPM). The amendment of NPK fertilizer plus pig manure (NPM) increased soil pH, and contents of soil organic carbon (SOC), total N and available P more effectively compared with NPK fertilizer plus crop residues. However, little is known how NPK fertilizer plus pig manure amendments regulate prokaryotic community structure.