Data from: Population structure, ancestral admixture, gene flow, and landscape association of blacklegged ticks during range expansion in the Midwestern U.S.

42 populations totalling 517 individuals of Ixodes scapularis from different spatial locations were sampled and sequenced to study the neutral variation, population structure, ancestral admixture, genetic connectivity, and landscape influences on gene flow. We began with genomic data preprocessing, variant calling, variant filtering and concordance check. Then we used the finalized dataset in variant call format (VCF) and spatial locations to conduct genetic distance statistics, isolation by distance modeling and calculate summary statstics. Further we used VCF and sample metadata to conduct Pincipal Component analysis and clustering analysis for understanding population structure and ancestral admixture. To understand region-wide gene flow connectivity, we conducted effective migration surface analysis and graph network analyses to visualize dispersal route and extent. Lastly, we processed landscape and ecological data to conduct landscape genomic analyses to understand the impact of landscape on gene flow, and visualized routes of dispersal across favorable environmental conditions.