Immunofluorescence data of 700 host cell proteins in Sars-CoV2 infected vs non-infected VeroE6 cells

The data set contains ≈ 75,000 confocal images of mixed Vero E6 cell populations (SARS-CoV2 infected vs non-infected). Cells had been infected with the virus for 1 h and fixed 24 hour post infection in 96 well plates. Using immunofluorescence (4 colours) cells were stained for the SARS-CoV2 virus (red), the endoplasmic reticulum (yellow) and nucleus (blue) – same for all images in the data set and used as reference markers. On top of this, 700 different target proteins were stained – one per well (green). Images were acquired with an Opera Phoenix microscope at 63X, using 9 FoV and 3 z-planes per protein target. IN the original study, intensity differences and spatial re-distribution of the target proteins were evaluated in infected vs the non-infected cells. The image resource was generated and described in detail in this research article: (to be added).

本数据集包含约75000张混合Vero E6细胞群的共聚焦显微镜图像，分为感染严重急性呼吸综合征冠状病毒2型（SARS-CoV-2）与未感染两组。细胞先被病毒感染1小时，于感染后24小时在96孔板中完成固定。所有图像均采用4色免疫荧光（immunofluorescence）染色方案，以SARS-CoV-2病毒（红色）、内质网（endoplasmic reticulum，黄色）与细胞核（蓝色）作为统一参考标记物。此外，每孔分别针对700种不同的目标蛋白进行绿色荧光染色，每孔对应单一目标蛋白。图像通过Opera Phoenix显微镜以63倍物镜采集，每个目标蛋白对应9个视场（Field of View, FoV）与3个Z轴层面。在原始研究中，研究者针对感染与未感染细胞内目标蛋白的强度差异及空间重分布情况开展了评估。本图像数据集由该研究生成，并将在相关研究论文中进行详细阐述（待补充）。