Effect of GK synthetic activator LY2121260 on wild type and mutant GST-GK fusion proteins.

GK activity was measured in standard conditions, as described in Material and Methods, in the absence and presence of 10 µM GK activator. Since LY2121260 was dissolved in a buffer containing DMSO, all assays contained a final concentration of 0.8% DMSO. Data represent means ± SEM of at least four separate experiments from two independent enzyme purifications. Statistical significance has been estimated comparing values in the presence of LY2121260 versus their corresponding values in the presence of buffer. (**) pp = 0.023.