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LXR-dependent gene expression in mouse bone marrow derived macrophages

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NIAID Data Ecosystem2026-03-11 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE118656
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Bone marrow derived macrophages were isolated from C57BL/6J (LXR positive) and LXRalpha +LXRbeta double knockout mice (DKO) that had been treated with vehicle, Kado2-lipid A (KLA, and inflammatory stimulus), acetylated LDL (AcLDL, a mimic of pro-atherogenic lipoprotein particles) and GW3965 (GW, a synthetic LXR agonist) for 17 hours. Following treatment RNA was isolated and RNA-Seq was performed. For each genotype (LXR positive and DKO) 3 mice/treatment were used for a total of 24 samples (6 mice x 4 treatment groups). Single-end 50 basepair sequencing was carried out at HudsonAlpha using a HiSeq 2500.
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2019-03-21
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