LXR-dependent gene expression in mouse bone marrow derived macrophages

Bone marrow derived macrophages were isolated from C57BL/6J (LXR positive) and LXRalpha +LXRbeta double knockout mice (DKO) that had been treated with vehicle, Kado2-lipid A (KLA, and inflammatory stimulus), acetylated LDL (AcLDL, a mimic of pro-atherogenic lipoprotein particles) and GW3965 (GW, a synthetic LXR agonist) for 17 hours. Following treatment RNA was isolated and RNA-Seq was performed. For each genotype (LXR positive and DKO) 3 mice/treatment were used for a total of 24 samples (6 mice x 4 treatment groups). Single-end 50 basepair sequencing was carried out at HudsonAlpha using a HiSeq 2500.