We measured global RNA levels using RNA-seq in Δcas3 E. coli cells with intact CRISPR arrays or cells with the CRISPR-I array deleted to determine if off-target events driven by endogenous spacers aff
Five different sgRNAs targeting mCherry gene (wild-type, without a spacer, and three truncated variants) were used to investigate the off-target effects of CRISPR interference. Total RNA sequencing da
The APC, KRAS, P53 and SMAD4 genes were mutated in human small intestinal stem cells and colon stem cells, which grow in vitro as epithelial organoids. Here we assessed candidate off-target sites for
