Hematopoietic Progenitor Cells of Different Donor Age

In this series we have analyzed the effect of donor age on the gene expression profile of human hematopoietic stem and progenitor cells (HPC). Cells were taken from umbilical cord blood (CB) or from G-CSF mobilized blood of healthy donors for allogeneic blood stem cell transplantation. Hematopoietic progenitor cells (HPC) were isolated within the CD34+ cell fraction from fresh human cord blood (CB) or from G-CSF mobilized peripheral blood (PB) as described in detail before (Wagner et al., Blood, 2004, 104:675-684; Wagner et al., Stem cells, 2005, 23:1180-1191; Wagner et al., 2007, 10:2638-2657). Differential gene expression was subsequently compared in the CD34+ samples of different donor age.