Long noncoding RNA expression profiles in intermittent parathyroid hormone induced cementogenesis

The aim of this study was to explore the involvement of long noncoding RNAs (lncRNAs) during intermittent parathyroid hormone (PTH) induced cementogenesis. OCCM-30 cells at 80% confluence were treated with 100 ng/ml PTH (dissolved in 0.1% acetic acid), while the control group was treated with 0.1% acetic acid. The treatment of PTH or acetic acid lasted for the first 6 hours in each cycle (one cycle represented 24 hours), then OCCM-30 cells were treated with fresh medium containing no PTH or acetic acid during the subsequent 18 hours in each cycle. After 2 cycles of PTH treatment, cells were harvested and tested. All the samples with three biological replicates were hybridized for each condition (PTH group and control group).