Identification and Differential Expression of MicroRNAs Associated with Fat Deposition in Wistar Rat Liver Using Solexa Sequencing

we analyzed the miRNAs expression in Wistar rat liver tissues, High glucose (HG)-induced NAFLD Wistar rats (NAFLD group, n=3) and normal Wistar rats (normal group, n=3). Results provided the first global miRNAs in the NAFLD Wistar rat livers, and expanded the miRNAs repertoire in normal Wistar rat livers, which should be useful for further investigation into the biological functions and evolution of miRNAs in rats and other species. These findings suggest an important role of miRNAs in liver fat deposition, and implicate the participation of miRNAs in the NAFLD pathophysiological processes.本研究针对Wistar大鼠肝组织中的微小核糖核酸（miRNAs）表达水平展开分析。实验设置两组受试对象：高糖（High Glucose, HG）诱导的非酒精性脂肪性肝病（Non-Alcoholic Fatty Liver Disease, NAFLD）模型Wistar大鼠（NAFLD组，n=3），与正常Wistar大鼠（正常组，n=3）。本研究结果首次获取了NAFLD模型Wistar大鼠肝脏的全范围miRNAs表达谱，并扩充了正常Wistar大鼠肝脏的miRNAs表达库，可为后续探究大鼠及其他物种中miRNAs的生物学功能与演化规律提供重要参考。上述研究结果表明，miRNAs在肝脏脂肪沉积过程中发挥关键作用，且提示miRNAs参与了NAFLD的病理生理进程。