Assessment of protein extraction and digestion efficiency of well-established shotgun protocols for heart proteomics

In this study, we evaluated three well-established sample preparation methods for shotgun proteomics, named: i) UREA; ii) sodium dodecyl sulfate (SDS); and iii) filter-aided sample preparation (FASP) protocols. We focused our analysis on protein extraction and digestion from heart left ventricle (LV) tissue.The three methods were compared by relative quantification using dimethyl labeling.