Comparison of gene expression with immunofluorescent chromosome banding and ChIP-seq of histone modifications in cell-cycle sorted lymphoblastoid and HeLa cells [array]

Metaphase chromosome staining was used to provide a high level overview of the pattern of histone modifications (H3K27ac, H3K27me3 and H3K4me3) at a single cell level in human lymphoblastoidand HeLa cells. A higher resolution view was achieved using ChIP-seq in cell-cycle sorted cells. These epigenomic haplotypes were related to various genomic features including gene and CpG island density as well as gene expression. A 6 array study using RNA extracted from 3 separate cultures each of human lymphoblastoid cells and HeLa cells