Microneedling-Assisted Delivery of Type III and Type XVII Collagen for Refractory Androgenetic Alopecia: A 24-Week Pilot Series

Supplementary Figure 1: A 52-year-old male with an 8-year history of androgenetic alopecia (AGA) had previously undergone a 3-year course of topical minoxidil and oral finasteride without significant improvement. He discontinued treatment and did not pursue further therapies until receiving a 24-week course of microneedling with type III collagen (COL3) and type XVII collagen (COL17). Serial photographs obtained at baseline, week 12, and week 24 demonstrated a marked improvement in hair density following treatment.
Supplementary Figure 2: A 51-year-old male with a 12-year history of androgenetic alopecia (AGA) underwent microneedling treatment with type III collagen (COL3) and type XVII collagen (COL17). Following 12 weeks of treatment, repigmentation of previously gray hair was observed, and newly grown hair exhibited pigmentation. Newly pigmented hairs are indicated within the dashed circles. (Scale bar = 2 mm.)
Supplementary Figure 3: A 53-year-old male with a 5-year history of androgenetic alopecia (AGA) underwent a 24-week course of microneedling treatment with type III collagen (COL3) and type XVII collagen (COL17). Pre-treatment and post-treatment high-frequency ultrasound images demonstrate an increase in both superficial dermal (SD) and deep dermal (DD) thickness, with no apparent change in the epidermal layer (E). (Scale bar = 1 mm.)
Supplementary Table 1: Scalp layer thickness measured by high-frequency ultrasound.
Supplementary Table 2：Hair density measured by trichoscopy.